TRANSOPTIC: High throughput all-optical single cell transcriptomics on a chip

The project at a glance

• Start date:
  01 August 2024

• Duration in months:
  36
  

• Funding:
  IAS Luxembourg / European Commission

• Principal Investigator(s):
  Dr. Markus LUDWIG
  
  

About

Single-cell transcriptomics is at the foundation of modern biological and medical research targeting functionalities of biological cells at the genetic level. The ability to snapshot the execution of genetic code allows to tailor medication, understand cell interactions as well as disease pathways. The state of the art relies on single-cell mRNA sequencing. The bottleneck in this technology is the high cost, low throughput and the need for lysing, i.e. impossibility to perform studies on live cells.

This project aims at rapid all-optical readout of cellular states at large scale based on high-sensitivity stimulated Raman scattering by integrating two emerging chip-scale technologies. This approach will enable high detection rates, low cost and robustness allowing even kinetic studies on live cells. This paradigm shift in cellular analysis not only overcomes current technological bottlenecks but also opens new horizons for dynamic, in-depth biological and neurological exploration.

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Actions grant agreement 101081455.

Organisation and partners

Faculty of Science, Technology and Medicine (FSTM)
Luxembourg Centre for Systems Biomedicine (LCSB)
Institute for Advanced Studies (IAS)
European Commission